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将真蓝(true blue)混悬液注入出生后7、14、28d大鼠眼球内,经过一定时间后,视神经内的轴突和少突胶质细胞被荧光标记。荧光强度在视神经眼球端强于视神经交叉端;出生后14～28d的幼鼠荧光标记明显强于出生后7d的幼鼠;不同年龄组的动物荧光标记普遍在注射荧光染料后的第5d显著增强。本研究表明,荧光染料可被视网膜的节细胞吸收,经轴突输送,然后,横向扩散到少突胶质细胞,扩散的通路可能是朗氏结旁区的轴胶连接。
The true blue suspension was injected into the eyeball of rats at 7, 14 and 28 days after birth. After a certain period of time, axons and oligodendrocytes in the optic nerve were fluorescently labeled. Fluorescence intensity was stronger at the optic nerve end of the optic nerve than at the cross of the optic nerve. The neonatal mice 14th to 28th day after birth were significantly more fluorescent than the 7th day after birth. Fluorescent labeling of animals of different age groups was significantly increased on the 5th day . This study shows that the fluorescent dye can be absorbed by the retinal ganglion cells, transported by the axon, and then diffused laterally into the oligodendrocytes. The diffuse pathway may be the axon junction of the Lanx node.