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通过对洪泽凹陷低熟原油中饱和烃化合物组合研究表明，它们能够较为有效和准确地确定低熟原油的生烃母质和沉积环境。在洪泽凹陷原油中，存在着典型半咸水—咸水环境的生物标志物组合，如Ｐｒ／Ｐｈ＜１，丰富的伽玛蜡烷，相对较高含量的Ｃ２９４－甲基甾烷，Ｃ２６甾烷，甲藻甾烷等。虽然洪泽凹陷具有近物源的沉积特征，但原油中的化合物组合仍表明半咸水—咸水环境的菌藻类微生物是低熟油的重要生烃母质。原油中高丰度的ｎＣ２２或ｎＣ２３正构烷烃，异构、反异构烷烃，Ｘ化合物，长链类异戊二烯烷基苯、甲藻甾烷等化合物的检出均证实这种生源输入的贡献。同时，原油中还检测到Ｃ３０未知三萜烷（ｕｃＴ）的存在，作者推测它与该环境形成低熟原油的某种特殊成烃母质有关
Through the study on the combination of saturated hydrocarbon compounds in low-mature crude oil of Hongze Sag, it is shown that they can determine the hydrocarbon generating parental and sedimentary environment of low-mature oil more effectively and accurately. Among the Hongze Sag crude oil, there are biomarker combinations of typical brackish-brackish environment such as Pr / Ph <1, rich gamma-wax, relatively high content of C294-methyl-sterane, Steranes, dinostenes and the like. Although the Hongze Sag has sedimentary characteristics of near-provenance, the combination of compounds in crude oil still indicates that the algae-like microorganisms in the brackish-brackish environment are important hydrocarbonogenic precursors for low-mature oil. The detection of nC22 or nC23 n-alkanes, isomeric, trans-isoparaffins, X compounds, long-chain isoprenoid alkylbenzenes, dinostenes and other compounds in high crude oil confirmed the import of contribution. At the same time, the presence of C30-unknown triterpenoid (ucT) was also detected in the crude oil, and the authors speculate that it is associated with a particular hydrocarbon-forming parent that forms the low-mature oil in the environment