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为了研究黄瓜果实采后的耐冷性,将‘津优1号’黄瓜(Cucumis sativus L.)果实在2℃下贮藏9d后,转移到20℃下放置2d,分别测定头部(花萼端)、中部、尾部(果梗端)3个部位的冷害指数、电导率、磷脂酶D(PLD)和脂氧合酶(LOX)活性、膜结合Ca2+含量。黄瓜果实的冷害最初在头部出现,再逐渐蔓延到中部和尾部。冷藏以后的黄瓜果实冷害指数、电导率、PLD活性、LOX活性均呈现为头部最高,中部次之,尾部最低。与膜结合的Ca2+含量则从黄瓜头部到尾部逐渐递增。结果表明,黄瓜果实从尾部到头部的耐低温能力逐渐降低,果实冷害程度与PLD和LOX活性提高和膜结合钙离子浓度下降显著相关。
In order to study the cold tolerance of cucumber fruits after harvest, the fruits of Cucumis sativus L. were stored at 2 ℃ for 9 days and then transferred to 20 ℃ for 2 days to measure the head (calyx end) Chilling injury index, conductivity, phospholipase D (PLD) and lipoxygenase (LOX) activity in three parts of the middle, tail (fruit stem end), membrane bound Ca2 + content. Chilling injury of cucumber fruit first appeared in the head, and then gradually spread to the middle and tail. After cold storage cucumber fruit chilling injury index, conductivity, PLD activity, LOX activity showed the highest head, the middle of the second, the tail of the lowest. The membrane-bound Ca2 + content gradually increased from head to tail of cucumber. The results showed that the cold tolerance of cucumber fruit from the tail to the head decreased gradually, and the degree of chilling injury was significantly correlated with the increase of PLD and LOX activity and the decrease of membrane-bound calcium ion concentration.