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作者在光学和电子显微镜下利用组织化学的方法,观察肌纤维细胞在掌挛缩病中的作用。材料和方法取21例掌挛缩病人的组织(男19例、女2例,均施行过部分筋膜切开术)和6例因其它原因而作手术病人的掌筋膜作为对照。作光镜检查者,在结节区和近侧筋膜索取标本,置入液氮内冰冻,在-20℃下切片,分别在 pH 为4.3、7.2和10.4的缓血酸胺(Tris)中孵育后,放入缓冲的 ATP 介质中作 ATP 酶染色。亦有用阿辛蓝或苏木紫染色。作电镜检查者,取1 mm~3的同上标本,用3%戊二醛固定。冰冻的切片在87℃的条件下置于含50 mg/6ml ATP 的盐酸 Tris 缓冲介质中孵育1小时,观察在 pH 4.3、7.2和10.4情况下的 ATP 酶活性。对照组的切片放在无 ATP 的相同基质中孵育。结果所有掌挛缩病人的结节中均见到肌纤维细
The authors used the method of histochemistry under optical and electron microscopes to observe the role of myofibroblasts in the treatment of contracture. MATERIALS AND METHODS Twenty-one cases of palmar contracture patients (19 males and 2 females, all underwent partial fasciotomy) and 6 cases of palm fascia for other reasons were selected as control. For light microscopy, specimens were taken from the nodal and proximal fascia, frozen in liquid nitrogen and sectioned at -20 ° C in Tris (pH 7.4, 7.2 and 10.4 respectively) After incubation, ATP is stained in buffered ATP medium. Also useful Alcian blue or hematoxylin staining. For electron microscopy, take 1 mm ~ 3 Ibid specimens, fixed with 3% glutaraldehyde. Frozen sections were incubated for 1 hour in Tris buffer containing 50 mg / 6 ml of ATP at 87 ° C and ATPase activity was observed at pH 4.3, 7.2 and 10.4. Control sections were incubated in the same substrate without ATP. Results All patients with contracture palm nodules seen in the thin fibers