论文部分内容阅读
生化实验室中测定血清铁的主要问题是敏感度差。使用铬天青B(chromazurol B)及十六烷基三甲基溴化铵(CTMA)与铁离子反应,生成一种深色的三元复合物,使敏感度提高数倍,从而解决了这个问题。显色的最适pH是4.6-5.5,光吸收最高峰在630nm。在0-80微克分子铁/升范围内符合比耳定律。在630nm的克分子消光系数是1.68×10~5升。克分子~(-1)·厘米~(-1)。试剂 (1)pH4.75缓冲液:溶解25克无水醋酸钠、110克氯化钠于重蒸馏水,约加8毫升浓冰醋酸(100%)校正pH后稀释到1升。 (2)显色剂:每升含0.3%CTMA90毫升,0.1%铬天青B铵盐60毫升,缓冲液850毫升。 (3)隐匿试剂:每100毫升含柠檬酸(一份结晶水)17克,柠檬酸钠(二份结晶水)35克。
The main problem with serum iron in biochemical laboratories is poor sensitivity. The use of chromazurol B and cetyltrimethylammonium bromide (CTMA) react with ferric ions to produce a dark ternary complex that increases sensitivity several fold, thus resolving this problem. The optimum pH for color development is 4.6-5.5 and the peak for light absorption is at 630 nm. Beer’s law is in the range of 0-80 micromoles iron / liter. The molecular extinction coefficient at 630 nm is 1.68 × 10 -5 liters. Molecule ~ (-1) · cm ~ (-1). Reagent (1) pH 4.75 Buffer: Dissolve 25 g of anhydrous sodium acetate, 110 g of sodium chloride in double distilled water and about 8 ml of concentrated glacial acetic acid (100%) to adjust the pH to 1 liter. (2) Color developer: 90 ml of 0.3% CTMA per liter, 60 ml of 0.1% chrome azure B ammonium salt and 850 ml of buffer. (3) Occult Reagents: Each 100 ml containing citric acid (a crystal water) 17 grams, sodium citrate (two crystal water) 35 grams.