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目的探讨红芪多糖(HPS)对糖尿病肾病(DN)db/db小鼠肾脏的保护机制。方法以12周龄雄性db/m小鼠10只作为正常组,同周龄雄性db/db小鼠50只随机分为HPS高、中、低剂量组和依那普利组、模型组,每组10只。HPS高、中、低剂量组分别给予HPS 400、200和100mg/(kg·d)灌胃;依那普利组给予依那普利10mg/(kg·d)灌胃;模型组、正常组给予等量生理盐水灌胃;各组均连续灌胃8周。采用免疫组织化学法和免疫印迹法检测各组小鼠肾组织血管内皮生长因子(VEGF)与色素上皮细胞衍生因子(PEDF)蛋白表达;RT-PCR法检测各组小鼠肾组织VEGF与PEDF mRNA表达。结果模型组较正常组VEGF mRNA及蛋白表达均上调(P<0.01),PEDF mRNA及蛋白表达均下调(P<0.01);与模型组比较,除HPS低剂量组外(P>0.05),其余各给药组VEGF mRNA及蛋白表达均下降(P<0.05或P<0.01),PEDF mRNA及蛋白表达均上升(P<0.05或P<0.01)。结论 HPS可通是过调节db/db小鼠肾组织中VEGF、PEDF蛋白和mRNA的表达,从而起到保护DN小鼠肾脏的作用。
Objective To investigate the protective mechanism of Hedysarum polysaccharide (HPS) on the kidney of diabetic nephropathy (DN) db / db mice. METHODS: Ten male 12-week-old db / m mice were used as normal control group, while 50 male db / db mice of the same age were randomly divided into HPS high, medium and low dose groups and enalapril group. Group of 10. The rats in the high, middle and low dose HPS groups were given intragastric administration of HPS at doses of 400, 200 and 100 mg / (kg • d), enalapril 10 mg / (kg • d) Given the same amount of saline gavage; each group were continuous gavage for 8 weeks. Immunohistochemistry and Western blotting were used to detect the expression of vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF) in renal tissue of each group; RT-PCR was used to detect the expression of VEGF and PEDF mRNA expression. Results Compared with the model group, the expression of VEGF mRNA and protein were up-regulated (P <0.01) and the expression of PEDF mRNA and protein were all decreased (P <0.01). Compared with the model group, except the HPS low dose group The expression of VEGF mRNA and protein were decreased in all groups (P <0.05 or P <0.01), and PEDF mRNA and protein expression were increased (P <0.05 or P <0.01). Conclusions HPS can regulate the expression of VEGF and PEDF protein and mRNA in the kidney of db / db mice and thus protect the kidneys of DN mice.