论文部分内容阅读
众所周知,带R—因子的大肠杆菌和绿脓杆菌产生的6’-N-乙酰转移酶(简称为AAC(6’))系构成对一些氨基糖甙类抗菌素耐药机制的组成部份;例如卡那霉素,卡那霉素B.3’、4’—二脱羟卡那霉素B(简称为DKB)和Ribostamycin可被上述酶所钝化;庆大霉素CIa和Sisomicin亦然。本文报导有关从绿脓杆菌制备的AAC(6)’对基质结构的某些要求。根据前文所述的方法制备绿脓杆菌GN315在100,000g离心分离
It is well-known that 6’-N-acetyltransferase (abbreviated as AAC (6 ’)) produced by R-factor-containing Escherichia coli and Pseudomonas aeruginosa forms part of the mechanism of resistance to some aminoglycoside antibiotics; for example, Kanamycin, kanamycin B.3 ’, 4’-dideoxykanamycin B (abbreviated as DKB) and Ribostamycin can be inactivated by the above enzymes; gentamicin CIa and Sisomicin are also known. This paper reports some of the requirements for matrix structure of AAC (6) ’prepared from Pseudomonas aeruginosa. Pseudomonas aeruginosa GN315 was prepared according to the method described above and centrifuged at 100,000 g