具有光驱动质子泵功能的嗜盐菌紫膜是一种被广泛研究的生物膜系统。利用毫秒级闪光动力学谱仪研究蜂毒不同突变体对紫膜质子泵功能的影响。实验采用对硝基苯酚（ｐ－ｎｉｔｒｏｐｈｅｎｏｌ）作为ＰＨ敏感染料来研究紫膜蛋白－菌紫质（Ｂａｃｔｅｒｉｏｒｈｏｄｏｐｓｉｎ，简称）的光反应和质子泵功能。在初步的实验中发现，在一定的温度范围内，光循环过程随着温度的升高而加快，质子泵功能却基本保持不变。另外，由于蜂毒突变体具有不同的插膜特性和不同的电荷量，通过比较它们对ＢＲ质子泵功能的影响，揭示了蜂毒小肽Ｃ端和Ｎ端的不同带电状态对质子泵功能所起的作用不同。实验结果有力地证实了蜂毒或其突变体与菌紫质蛋白具有直接的相互作用，这种相互作用的强弱与蜂毒突变体电荷的多少密切相关 Halophilic violet membrane with light-driven proton pump function is a widely studied biofilm system. Effects of different mutants of venom on the function of the proton pump in the purple membrane were studied by millisecond flash kinetic spectroscopy. The experiment used p-nitrophenol as PH-sensitive dye to study the photoproduction and proton pump function of the bacteriorhodopsin (abbreviated). In preliminary experiments, it was found that in a certain temperature range, the photocyclization process accelerated with the increase of temperature, and the proton pump function remained basically unchanged. In addition, because of the different intercalator properties and different charge amounts, the mutants of bee venom showed that the different charged states of C-terminal and N-terminal of the bee venom peptide on proton pump function by comparing their effects on the function of BR proton pump Different role. The experimental results strongly confirmed that bee venom or its mutants have a direct interaction with bacteriorhodopsin, and the strength of this interaction is closely related to the charge of the bee venom mutant