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目的:探讨拉曼光谱用以鉴别正常精子和死精子的可行性。方法:以10例捐精者正常精子为实验组,5例死精子为对照组。伊红染色法鉴别死活精子,分别按照形态学对精子核仁、顶体、颈部进行拉曼光谱扫描,比较研究组和对照组拉曼光谱强度。结果:精子的拉曼光谱主要由500~650/cm、1 000~1 200/cm 2个区域组成,峰强集中在550/cm及1 100/cm左右,不同扫描位点的拉曼光谱无统计学差异(P>0.05)。死精子在拉曼光谱强度上明显强于正常精子:正常精子强度普遍<300 IU,死精子强度>400 IU。结论:拉曼光谱可对精子的存活能力提供新的评估方法,并为进一步筛选正常精子用于辅助生殖技术开拓新思路。
Objective: To explore the feasibility of Raman spectroscopy to identify normal and dead sperm. Methods: Normal sperms from 10 donors were used as experimental group and 5 dead sperm as control group. Eosin staining of live and dead sperm were identified, respectively, according to morphological sperm nuclei, acrosome, neck Raman spectroscopy scan comparison of the Raman intensity of the group and the control group. Results: The Raman spectra of sperm mainly consisted of 500 ~ 650 / cm and 1000 ~ 1 200 / cm 2 regions with peak intensities at 550 / cm and 1 100 / cm. The Raman spectra of different scanning sites Statistical difference (P> 0.05). Dead sperm is significantly stronger than normal sperm in Raman intensity: <300 IU for normal sperm and 400 IU for dead sperm. Conclusion: Raman spectroscopy can provide a new evaluation method for sperm viability and explore new ideas for further screening of normal sperm for assisted reproductive technology.