采用小鼠骨髓细胞体外琼脂单层培养法,在直径30mm培养皿中加入CSF0.1ml,后加入不同浓度的碳酸锂溶液,在37℃恒温培养6天,显微镜下计数CFU—GM集落数量.结果表明,纯碳酸锂浓度在2.0～5.0mEq/L范围内,CFU-GM产率均有不同程度的增长,浓度为3.0mEq/L时,培养的细胞生长最好,为正常对照组产率的155.43％,P<0.01。 In vitro agar monolayer culture of mouse bone marrow cells was used to add CSF0.1ml into a 30mm diameter culture dish, then different concentrations of lithium carbonate solution were added and incubated at 37 ° C for 6 days, and the number of CFU-GM colonies was counted under a microscope. The results showed that the yields of CFU-GM increased with the concentration of pure lithium carbonate in the range of 2.0-5.0 mEq / L. When the concentration was 3.0 mEq / L, the growth of the cells was the best, which was the normal control group 155.43%, P <0.01.