近年来,国内外在实施体外授精和人工授精时,为改善精液质量,从精液中分离并回收优质精子,常常采用精液静置法(Layering method)。用此方法从正常或接近正常的精液中,分离回收优质精子是极其有效的。但对粘稠性高,液化时间延长或精子活动无力的精液,回收精子的效果较差。1984年Leung改用搅拌离心法,获得较好的效果。1988年吉野和男用精子上游(swin up)洗涤浓缩法、使回收精子活动率提高2倍,授孕指数提高1.3倍。妊娠率亦有显著上升。1989年我们就精子上游洗涤浓缩法中的回收精子活动率与精液 In recent years, at home and abroad in the implementation of in vitro fertilization and artificial insemination, in order to improve the quality of semen, semen from the semen and recovery of high-quality sperm, often using the semen method (Layering method). Using this method to separate and recover high quality sperm from normal or near-normal sperm is extremely effective. However, high viscosity, prolonged liquefaction or sperm motility semen, sperm recovery less effective. 1984 Leung switched to stirring centrifugation, get better results. In 1988 Yoshino and male swin up washing concentration method, so that sperm recovery activity increased by 2 times, pregnancy index increased 1.3 times. The pregnancy rate also increased significantly. 1989, we sperm recovery and enrichment method upstream of the recovery rate of sperm motility and semen