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目的观察不同浓度尿酸对人骨髓间充质干细胞(hBMSCs)成骨分化过程中核心结合因子α1(Cbfα1/Runx2)表达变化的影响。方法以体外培养的健康成年hBMSCs为研究对象,分为5个组,分别为对照组(完全培养基组)和加入不同浓度尿酸(0 mmol/l、0.2 mmol/l、0.4 mmol/l、0.8mmol/l)的成骨诱导组,通过倒置显微镜观察细胞形态,碱性磷酸酶染色和茜素红染色鉴定细胞。在干预诱导第7天和第14天行RT-PCR检测Cbfα1/Runx2的表达。结果碱性磷酸酶染色和茜素红染色结果均阳性,表示诱导后细胞为成骨细胞。RT-PCR结果表明,对照组各时间点均无Cbfα1/Runx2表达,尿酸培养组随尿酸浓度增加和时间的延长,Cbfα1/Runx2表达逐渐增强,呈现时间依赖性和浓度依赖性。结论尿酸可能通过促进Cbfα1/Runx2的表达,从而促进hBMSCs向成骨细胞分化。
Objective To observe the effects of different concentrations of uric acid on the expression of Cbfα1 / Runx2 during the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs). Methods Healthy adult hBMSCs cultured in vitro were divided into five groups: control group (complete medium group) and uric acid (0 mmol / l, 0.2 mmol / l, 0.4 mmol / l, mmol / l). The cells were identified by inverted microscope, cell morphology, alkaline phosphatase staining and alizarin red staining. RT-PCR was performed to detect the expression of Cbfα1 / Runx2 on day 7 and day 14 of intervention induction. Results Alkaline phosphatase staining and alizarin red staining were positive, indicating that the induced cells were osteoblasts. The results of RT-PCR showed that there was no Cbfα1 / Runx2 expression in control group at each time point. The expression of Cbfα1 / Runx2 in uric acid culture group was increased with the increase of uric acid concentration and time, showing a time-dependent and concentration-dependent manner. Conclusion Uric acid may promote the differentiation of hBMSCs into osteoblasts by promoting the expression of Cbfα1 / Runx2.