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用结肠癌细胞系Ls_(174)t免疫BALB/c小鼠,获得的两株单克隆抗体CL-3及CL-4,分属于IgG2a及IgM。用免疫组化SABC方法对石蜡包埋组织切片检测,CL-3在大肠癌的表达率为88.9％,在胰腺癌及胃癌的表达率为100％及91.6％。此外在食道癌、肺癌及膀胱癌亦有表达(66.6～72.7％)。而在正常组织,大肠表达29.6％,肺支气管粘液腺66％,其他皆为阴性。CL-4在大肠癌、胰腺及胃癌的表达率分别为88.9％,75％及69.2％。此外,在食道癌及
BALB/c mice were immunized with the colon cancer cell line Ls_(174)t. Two monoclonal antibodies CL-3 and CL-4 were obtained, belonging to IgG2a and IgM. Paraffin-embedded tissue sections were detected by immunohistochemical SABC method. The expression rate of CL-3 in colorectal cancer was 88.9%. The expression rate of CL-3 in pancreatic cancer and gastric cancer was 100% and 91.6%. In addition, esophageal cancer, lung cancer, and bladder cancer were also expressed (66.6 to 72.7%). In normal tissues, 29.6% in the large intestine and 66% in the lung bronchial mucinous glands. All were negative. The expression rates of CL-4 in colorectal cancer, pancreas, and gastric cancer were 88.9%, 75%, and 69.2%, respectively. In addition, in esophageal cancer and