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目的:通过检测缺血再灌注大鼠血清中CK-MB水平、心肌梗死面积等指标,探讨心脑通络液预处理联合针刺后处理对大鼠缺血再灌注心肌细胞的影响。方法:健康轻洁级Wistar大鼠36只,随机分为6组:正常组、假手术组、缺血再灌注组、心脑通络液预处理组、针刺后处理组、心脑通络液预处理组+针刺后处理组。以结扎大鼠左冠状动脉前降支(LAD)30min,再灌注120min建立缺血再灌注损伤模型。检测大鼠CK-MB水平、TTC法检测大鼠心肌梗死面积。结果:心脑通络液预处理组、针刺后处理组、心脑通络液预处理+针刺后处理组较缺血再灌注组大鼠CK-MB水平明显下降,心肌梗死面积减小,其中心脑通络液预处理+针刺后处理组与心脑通络液预处理组、针刺后处理组比较差异具有显著性(P<0.05)。结论:心脑通络液预处理、针刺后处理可改善缺血再灌注大鼠血清中CK-MB水平,减小心肌梗死面积,说明其具有减轻缺血再灌注损伤的作用,心脑通络液预处理联合针刺后处理效果优于心脑通络液预处理、针刺后处理。
Objective: To investigate the effect of Xinnaotongluo preconditioning and acupuncture postconditioning on myocardial ischemia / reperfusion (I / R) cardiomyocytes in rats by detecting CK-MB and myocardial infarct size in ischemia-reperfusion rats. Methods: Thirty-six healthy Wistar rats were randomly divided into 6 groups: normal group, sham operation group, ischemia-reperfusion group, Xinnaotongluo preconditioning group, acupuncture postconditioning group, Liquid pretreatment group + acupuncture post-treatment group. The left anterior descending coronary artery (LAD) was ligated for 30 minutes and reperfused for 120 minutes to establish a model of ischemia-reperfusion injury. The levels of CK-MB in rats were detected, and the area of myocardial infarction in rats was detected by TTC method. Results: The levels of CK-MB in the Xinnaotongluo preconditioning group, the acupuncture treatment group, the Xinnaotongluo preconditioning group and the acupuncture treatment group were significantly lower than those in the ischemia-reperfusion group, and the myocardial infarct size was decreased (P <0.05). The difference between Xinnao Tongluo pretreatment + acupuncture treatment group and Xinnaotongluo pretreatment group and acupuncture treatment group was significant (P <0.05). Conclusion: Xinnaotongluo preconditioning and acupuncture postconditioning can improve the level of CK-MB in serum and decrease the area of myocardial infarction in ischemia-reperfusion rats, indicating that it has the effect of reducing ischemia-reperfusion injury. The pretreatment of colloidal fluid combined with acupuncture postconditioning is better than that of Xinnaotongluo pretreatment and acupuncture postconditioning.