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The dose-dependent cytotoxicity effect on human hepatocyte(HL-7702 cells) induced by “naked” Fe3O4 nanoparticles was assessed through cell viabilities and lactate dehydrogenase(LDH) activities. Three important oxi dative indexes of the cells by glutathione peroxidase(GSH-Px), superoxide dismutase(SOD) and malondialde hyde(MDA) were determined. The good correlation of the cell viabilities with their GSH-Px, SOD and MDA levels indicated that the cytotoxicity is related to activation of oxidative stress induced by Fe3O4 nanoparticles. The oxida tive stress also leads to corresponding DNA damage in a similar dose-dependent manner, followed by the changes of cell cycle and cell apoptosis. Such work provides important experimental data for the safety evaluation of superpa ramagnetic Fe3O4 nanoparticles.
The dose-dependent cytotoxicity effect on human hepatocyte (HL-7702 cells) induced by “naked ” Fe3O4 nanoparticles was assessed through cell viabilities and lactate dehydrogenase (LDH) activities. Three important oxi dative indexes of the cells by glutathione peroxidase The good correlation of the cell viabilities with their GSH-Px, SOD and MDA levels indicated that the cytotoxicity is related to activation of oxidative stress induced by Fe3O4 nanoparticles . The oxida tive stress also leads to corresponding DNA damage in a similar dose-dependent manner, followed by the changes of cell cycle and cell apoptosis. Such work provides important experimental data for the safety evaluation of superparamagnetic Fe3O4 nanoparticles.