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在中成药生产中以土大黄或大黄中掺有土大黄投料现象常有,为此我们用TLC和TLCS法检出中成药中土大黄。一、TLC检查法1.供试液制备:取大黄、土大黄各2g,另取各供试样品(见表)适量(相当于含大黄2g),粉成细粉(蜜丸剪碎即可)。加乙醇20ml冷浸2hr,取上清液供点样用。2.TLC试验:取土大黄甙,土大黄和大黄对照液及供试液各2μl,点于经105℃活化半小时的硅胶G(青岛海洋化工厂)板上,以乙酸乙酯,甲醇(8:2)上行展开。展距15cm,取出挥干溶剂,于紫外灯下观察,结果如图。
In the production of proprietary Chinese medicines, rhubarb or rhubarb is often mixed with rhubarb, and we often use TLC and TLCS to detect Chinese rhubarb. First, TLC inspection method 1. Preparation of test solution: take rhubarb, rhubarb each 2g, another take each test sample (see table) amount (equivalent to rhubarb 2g), powder into a fine powder (pear balls can be broken ). Ethanol 20ml cold soak 2hr, take the supernatant for sample use. 2. TLC test: Take 2 μl each of rhubarb, rhubarb and rhubarb control solution and test solution, and spot them on silica gel G (Qingdao Marine Chemical Factory) plate that has been activated at 105°C for half an hour, with ethyl acetate and methanol ( 8:2) Upwards. The exhibition distance was 15cm. The solvent was removed and observed under a UV lamp. The result is shown in the figure.