对真核生物DNA序列中控制转录成分进行鉴定的主要方法是诱变DNA序列中转录起始部位的邻近部分,然后将改变的模板重新引入细胞,或者加至某个实验体系(如体外转录系统),对该膜板进行试验,经过一段时间后,测定RNA或者蛋白质的生成量,据此推断序列改变对启动子作用强度的影响。这些研究表明,虽然编码各种蛋白质基因的启动子顺序各不相同,但他们部是按照一种共同的模式编组而成的。 The primary method of identifying transcriptional components in eukaryotic DNA sequences is by mutating adjacent portions of the transcriptional start site in the DNA sequence and reintroducing the altered template into the cell or into a laboratory system such as an in vitro transcription system ) Was used to test the membrane. After a certain period of time, the amount of RNA or protein produced was measured, and the effect of the sequence change on the strength of the promoter was inferred. These studies show that although the sequences of promoters that encode various protein genes vary, they are grouped together in a common pattern.