对广州市农贸市场中销售的大豆进行转基因大豆的初步筛查,以检测在广州市场是否有转基因大豆流通和销售,为相关标识和监管提供实验依据。根据转基因大豆内参凝集素Lectin基因及外源基因CaMV35S、NOS及EPSPS基因序列设计4对引物,对收集的大豆样品提取其基因组DNA后进行PCR检测。结果在所收集的14份大豆样品中检测出1份转基因大豆,表明在广州农贸市场中存在转基因大豆,并在市场流通,提醒相关部门需根据国家规定需要加强监管力度。 Conduct preliminary screening of soybeans for soybean sold in farmers market in Guangzhou to test whether there is circulation and sales of genetically modified soybeans in Guangzhou market and provide experimental evidence for related labeling and regulation. Four pairs of primers were designed according to Lectin gene and CaMV35S, NOS and EPSPS gene sequences of GM soybean lectin gene. The genomic DNA was extracted from the collected soybean samples for PCR detection. Results One copy of GM soy was detected in 14 soybean samples collected, indicating that GM soybean exists in Guangzhou farmers’ market and circulated in the market, reminding relevant departments that they need to strengthen their supervision in accordance with state regulations.