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目的:应用牛磺酸保护失血性休克再灌注后肠粘膜屏障功能。方法:24只家兔随机分为三组:牛磺酸治疗组(A组),单纯休克组(B),假手术组(C)。以硫代巴比妥酸比色法和原子吸收分光光度法测末端回肠粘膜MDA和组织钙含量,并取体循环血做细菌培养。结果:B组肠粘膜MDA和组织钙含量有明显升高,而A组变化无显著性;再灌注半小时后B组细菌移位率明显高于C组(P<0.05);光镜和电镜观察表明B组肠粘膜损伤明显重于A和C组。结论:牛磺酸能保护失血性休克再灌注后肠粘膜屏障功能,这可能与牛磺酸能清除氧自由基,防止钙超载有关。
Objective: To protect the intestinal mucosal barrier function after hemorrhagic shock and reperfusion with taurine. Methods: Twenty-four rabbits were randomly divided into three groups: taurine treatment group (A group), shock alone group (B) and sham operation group (C). The end-ileal mucosa MDA and tissue calcium content were measured by thiobarbituric acid colorimetric method and atomic absorption spectrophotometry, and bacterial blood was collected for bacterial culture. Results: The content of MDA and tissue calcium in group B were significantly increased, while the change in group A was not significant. The bacterial translocation rate in group B was significantly higher than that in group C after half an hour of reperfusion (P <0.05) Observation showed that intestinal mucosal injury in group B was significantly heavier than in groups A and C. CONCLUSION: Taurine can protect the intestinal mucosal barrier function after hemorrhagic shock and reperfusion, which may be related to taurine’s ability to scavenge oxygen free radicals and prevent calcium overload.