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目的:建立人血浆中氨氯地平浓度的测定方法,以考察其人体内药物动力学。方法:采用C_(18)反相柱(250 mm×4.6 mm,5μm),流动相为10 mmol·L~(-1)醋酸铵缓冲液(pH 3.5)-甲醇(20:80);流速:1.0 ml·min~(-1);检测离子:氨氯地平,m/z 341.2;内标:非洛地平,m/z 406.0。结果:氨氯地平回归方程Y=0.176C-0.004,权重系数w=1/C~2,r=0.9986。线性范围为0.12～11.9 ng·ml~(-1),最低检测浓度为0.12 mg·ml~(-1),高、中、低3个浓度的相对回收率分别为104.8%,98.7%,94.7%,日内、日间RSD均小于15%(n=5)。结论:该法灵敏、准确、快速,可用于血浆氨氯地平的浓度测定。
Objective: To establish a method for the determination of amlodipine in human plasma in order to investigate its pharmacokinetics in human. Methods: The mobile phase consisted of 10 mmol·L -1 ammonium acetate buffer (pH 3.5) and methanol (20:80) with a C 18 reversed-phase column (250 mm × 4.6 mm, 5 μm) 1.0 ml · min -1; detection ion: amlodipine, m / z 341.2; internal standard: felodipine, m / z 406.0. Results: Amlodipine regression equation Y = 0.176C-0.004, weight coefficient w = 1 / C ~ 2, r = 0.9986. The linear range was from 0.12 to 11.9 ng · ml -1, and the lowest concentration was 0.12 mg · ml -1. The relative recoveries of high, medium and low concentrations were 104.8%, 98.7%, 94.7 %, Day and day RSD were less than 15% (n = 5). Conclusion: The method is sensitive, accurate and rapid and can be used to determine the concentration of amlodipine in plasma.