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目的观察告达庭对大鼠神经胶质瘤C6细胞增殖和迁移能力的影响,并初步探讨其内在的分子机制。方法 MTT法检测告达庭对C6细胞存活力的影响;流式细胞术检测细胞周期的变化;细胞划痕实验及Transwell迁移小室检测告达庭对C6细胞体外迁移能力的抑制作用;免疫印迹实验检测告达庭对C6细胞内β-catenin、Survivin以及细胞周期相关蛋白CyclinD1和Cdk4蛋白表达的影响。结果告达庭能够剂量依赖性地抑制C6细胞的增殖并阻滞细胞于G1期,其机制与下调细胞周期素CyclinD1和Cdk4的表达相关;告达庭给药后,C6细胞的迁移能力明显受到抑制,同时β-catenin及其下游因子Survivin的表达呈显著下降趋势。结论告达庭具有抑制C6细胞的增殖和体外迁移能力的作用,其机制可能与抑制β-catenin蛋白及Survivin的表达相关。
OBJECTIVE: To observe the effects of procuratorate on the proliferation and migration of C6 glioma cells in rats and to explore its underlying molecular mechanism. Methods MTT assay was used to detect the effect of procampin on the viability of C6 cells. Flow cytometry was used to detect cell cycle changes. Cell scratch assay and Transwell migration chamber were used to detect the inhibitory effect of ADP on C6 cells in vitro. Western blotting To detect the expression of PDT on C6 cells in the β-catenin, Survivin and cell cycle-related protein CyclinD1 and Cdk4 protein expression. Results Tumor cells can inhibit the proliferation of C6 cells in a dose-dependent manner and arrest the cells in G1 phase, the mechanism of which is related to the down-regulation of CyclinD1 and Cdk4 expression. The migration ability of C6 cells is obviously affected Inhibition, while β-catenin and its downstream factor Survivin expression showed a significant downward trend. Conclusion Aspartame has the inhibitory effect on the proliferation and migration of C6 cells in vitro. The mechanism may be related to the inhibition of the expression of β-catenin and Survivin.