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目的探讨性别差异对脓毒症大鼠肝脏组织核因子-kB(NF-kB)活化的影响。方法取40只清洁级Wistar大鼠,雌雄各半,采用腹腔注射内毒素(LPS)5mg/kg制作脓毒症大鼠模型,利用凝胶阻滞迁移分析方法(EMSA)检测肝脏组织NF-kB的活化情况,同时检测大鼠血清中丙氨酸氨基转移酶(ALT),肿瘤坏死因子-α(TNF-α)以及雌二醇(E2)含量。结果正常雌雄性大鼠肝脏组织有NF-kB的微弱活化,同时血清中TNF-α及ALT含量较少,各项指标在两组大鼠间差异无显著性(P＞0．05)；脓毒症状态下各项指标明显升高,但是雌性大鼠各项指标的变化明显低于雄性(P＜0．01)；相关分析表明,雌雄性脓毒症大鼠肝脏组织NF-kB的活化以及血清中TNF-α,ALT含量均与相应组别大鼠血清中E2含量呈显著负相关(P＜0．05)。结论脓毒症大鼠肝脏组织NF-kB的活化及血清中TNF-α,ALT含量存在性别差异,内源性雌激素可能介导了对雌性脓毒症大鼠肝脏的保护作用。
Objective To investigate the effect of gender differences on the activation of nuclear factor-kB (NF-kB) in the liver of septic rats. Methods Forty Wistar rats of clean grade were divided into male and female rats. The model of sepsis was induced by intraperitoneal injection of lipopolysaccharide (LPS) 5 mg / kg. EMSA was used to detect the expression of NF-kB The levels of alanine aminotransferase (ALT), tumor necrosis factor-α (TNF-α) and estradiol (E2) in serum of the rats were detected. Results The normal tissues of the male and female rats were weakly activated by NF-κB, while the contents of TNF-α and ALT in the serum were less. There was no significant difference in the indexes between the two groups (P> 0.05) The indicators of the disease were significantly increased, but the changes of the indexes in the female rats were significantly lower than those in the male rats (P <0.01). Correlation analysis showed that the activation of NF-κB in the liver tissue of male and female sepsis rats (P <0.05). There was a significant negative correlation between serum TNF-αand ALT levels and serum E2 levels in the corresponding groups (P <0.05). Conclusion The activation of NF-κB in the liver tissue of septic rats and the serum levels of TNF-α and ALT have gender differences. The endogenous estrogen may mediate the protective effect on the liver of rats with sepsis.