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目的探讨多氯联苯118(PCB118)对大鼠甲状腺功能及自身免疫反应的影响。方法Wistar大鼠40只均分为四组:A组为空白对照;B、C、D组分别给予PCB118 10、100、1000μg.kg-1.d-1腹腔注射,每周5次。13周后检测血清游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、促甲状腺激素(TSH)、甲状腺球蛋白(TG)、甲状腺球蛋白抗体(TGAb)、过氧化物酶抗体(TPOAb)水平,甲状腺组织过氧化物酶(TPO)mRNA表达水平,观察甲状腺组织病理学改变。结果甲状腺组织病理学检查显示B、C、D组均可见滤泡腔塌陷、上皮脱落、间质纤维化、小血管增生,B、C组可见间质淋巴细胞浸润。随着PCB118剂量增加,B、C、D组血清FT3、FT4、TG浓度逐渐降低;D组FT3浓度明显低于A、B组(P<0.05);B、C、D组血清FT4、TSH浓度明显低于A组(P<0.05);C、D组血清TG水平明显低于A组(P<0.05)。与A组比较,B、C组血清TGAb、TPOAb浓度显著升高(P<0.05);C组TPO mRNA表达水平明显高于A组(P<0.05)。结论 PCB118可导致大鼠甲状腺组织形态和功能异常,激活甲状腺自身免疫反应。
Objective To investigate the effects of PCB118 on thyroid function and autoimmune reaction in rats. Methods Forty Wistar rats were divided into four groups: group A was blank control; group B, C, D were given PCB118 10,100,1000μg.kg-1.d-1 intraperitoneal injection 5 times a week. Thirteen weeks later, serum FT3, FT4, TSH, TGAb, (TPOAb), thyroid tissue peroxidase (TPO) mRNA expression levels were observed thyroid histopathological changes. Results Thyroid histopathology showed that follicular cavity collapse, epithelial shedding, interstitial fibrosis and small vessel hyperplasia were observed in group B, C and D, and interstitial lymphocyte infiltration in group B and C were observed. The concentrations of FT3, FT4 and TG in serum of group B, C and D decreased gradually with the increase of dose of PCB118. The FT3 concentration of group D was significantly lower than that of group A and B (P <0.05) (P <0.05). The level of serum TG in group C and D was significantly lower than that in group A (P <0.05). Compared with group A, serum concentrations of TGAb and TPOAb in group B and group C were significantly increased (P <0.05). The expression of TPO mRNA in group C was significantly higher than that in group A (P <0.05). Conclusion PCB118 can cause abnormal thyroid morphology and function in rats and activate thyroid autoimmune reaction.