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Cp G ODN可增强疫苗特异性免疫反应,但是它在动物体内易降解和吞噬率低的特性降低其作为免疫佐剂的效果。本研究尝试用葡聚糖-精胺(DSP)包裹Cp G ODN,保护其免受核酸酶降解,提高细胞吞噬率。所合成DSP包裹Cp G ODN形成DSP-Cp G ODN复合物,体外刺激脾脏淋巴细胞,ELISA法测量细胞培养上清中白介素12(IL-12)含量来分析复合物是否可更有效地激活免疫细胞。结果显示所合成DSP可完全包裹Cp G ODN形成有效粒径为500 nm的颗粒并降低核酸酶对Cp G ODN降解速率。在最佳包裹条件下DSP-Cp G ODN复合物所刺激的脾脏淋巴细胞IL-12分泌水平相比单独Cp G ODN组有15.5倍增加(p<0.001)。本研究表明DSP可望作为Cp G ODN输送载体,增强佐剂效果。
CpG ODN enhances vaccine-specific immune responses, but its low rate of degradation and phagocytosis in animals reduces its effectiveness as an adjuvant. In this study, we tried to encapsulate CpG ODN with dextran-spermine (DSP) to protect it from nuclease degradation and increase phagocytosis rate. The synthesized DSP encapsulated CpG ODN to form DSP-Cp G ODN complex, stimulated splenic lymphocytes in vitro, and the content of interleukin 12 (IL-12) in the cell culture supernatant was measured by ELISA to analyze whether the complex can activate immune cells more effectively . The results showed that the synthesized DSP could completely encapsulate CpG ODN to form particles with an effective particle size of 500 nm and reduce the degradation rate of CpG ODN by nucleases. IL-12 secretion of splenic lymphocytes stimulated by DSP-Cp G ODN complex under optimal conditions was increased 15.5 fold (p <0.001) compared to CpG ODN alone. This study shows that DSP is expected to serve as a carrier for CpG ODN delivery and enhances the adjuvant effect.