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目的探讨As2O3与5-氟尿嘧啶(5-FU)联合应用对肾癌细胞786-0细胞株细胞增殖、细胞凋亡及X连锁凋亡抑制蛋白(XIAP)表达的影响。方法取对数生长期786-0细胞,MTT比色法检测As2O3和/或不同浓度5-FU对细胞生长抑制作用;流式细胞仪检测细胞凋亡率;RT-PCR法及蛋白质印迹法检测XIAP mRNA表达及XIAP蛋白质表达的变化。结果不同浓度的5-FU分别作用786-0细胞后,细胞生长受到抑制,但无明显的时间、浓度相关性;2μmol/L As2O3与不同浓度5-FU联合应用对786-0细胞的增殖抑制率随作用时间、药物浓度的增加逐渐升高,与单药相比较(P<0.01),两药相互作用系数显示为协同作用。48h检测2μmol/L As2O3与不同浓度5-FU联合用药处理的786-0细胞的凋亡率明显增加,与单药相比(P<0.01);XIAP mRNA表达明显减少,与单药相比(P<0.01);2μmol/L As2O3与400μg/ml 5-FU联合应用48h XIAP蛋白表达亦明显减少,与单药相比(P<0.01)。结论 As2O3联合5-FU可以明显提高肾透明细胞癌的化疗敏感性,抑制786-0细胞增殖,诱导肿瘤细胞凋亡,这一作用与抑制786-0细胞XIAP基因的表达有关。
Objective To investigate the effects of As2O3 and 5-fluorouracil (5-FU) on cell proliferation, apoptosis and X-linked inhibitor of arrest protein (XIAP) expression in 786-0 human renal cell carcinoma cell line. Methods 786-0 cells in logarithmic growth phase were treated with As2O3 and / or different concentrations of 5-FU by MTT colorimetric assay. Flow cytometry was used to detect the apoptosis rate. RT-PCR and Western blotting XIAP mRNA expression and XIAP protein expression changes. Results After 786-0 cells were treated with different concentrations of 5-FU, the cell growth was inhibited, but there was no significant correlation between time and concentration. The proliferation of 786-0 cells treated with 2μmol / L As2O3 and different concentrations of 5-FU was inhibited The rate increased gradually with the increase of the drug concentration, and compared with the single drug (P <0.01), the interaction coefficient of the two drugs showed a synergistic effect. Compared with single drug (P <0.01), the apoptosis rate of 786-0 cells treated with 2μmol / L As2O3 and different concentrations of 5-FU was significantly increased at 48h (P <0.01) P <0.01). Compared with single drug, the expression of XIAP in 2μmol / L As2O3 and 400μg / ml 5-FU group was significantly decreased 48h (P <0.01). Conclusion As2O3 combined with 5-FU can significantly improve the chemosensitivity of renal clear cell carcinoma, inhibit the proliferation of 786-0 cells and induce the apoptosis of tumor cells, which is related to the inhibition of the expression of XIAP in 786-0 cells.