研究了邻 {2 - [α- (2 -羟基 - 5-磺基苯偶氮 ) -亚苄基 ]-肼基 }苯甲酸 (锌试剂 ,ZCN)与 L-赖氨酸 (L- Lys)复合物的吸光光度测定基本条件 ,在 p H 1 .9的缓冲溶液中 ,ZCN与 Lys结合生成紫红色复合物 ,最大吸收波长在 60 5nm处 ,标准工作曲线在 5.47× 1 0 -6～ 1 .64× 1 0 -4 mol· L-1范围内呈线性关系。在最大吸收峰处比锌试剂本身红移 1 45nm,复合物的表观摩尔吸光系数 ε为 2 .5×1 0 4 。同时 ,研究了离子强度对反应体系的影响 ,ZCN作为光谱探针与 Lys的结合数 (Zinc reagent) (Zinc reagent) and L-lysine (L-Lys) The basic conditions for the determination of the absorbance of the complex, in p H 1 .9 buffer solution, ZCN and Lys bond to form a purple complex, the maximum absorption wavelength at 60 5nm, the standard operating curve of 5.47 × 10-6 ~ 1 .64 × 10 -4 mol · L-1 in the range of linear relationship. At the maximum absorption peak red shift than the zinc reagent itself 1 45nm, the apparent molar absorptivity ε complex was 2.5 × 10 4. At the same time, the influence of ionic strength on the reaction system was studied. The binding number of ZCN as spectral probe to Lys