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采用蛭石栽培,在100mmol·L-1NaCl胁迫下,对耐盐性不同的2个菜用大豆[Glycinemax(L.)Merr.]品种结荚期干物质积累、单株产量及叶片游离态多胺(PAs)水平的变化进行了研究。结果表明:NaCl胁迫显著降低了菜用大豆植株干重及单株产量,但耐盐品种“绿领特早”的降幅低于盐敏感品种“理想高产95-1”;与“理想高产95-1”相比,“绿领特早”叶片在整个NaCl胁迫期间均维持了相对较低的H2O2含量、游离态腐胺(Put)含量及较高的游离态亚精胺(Spd)含量,在胁迫6～15d期间维持了相对较高的游离态精胺(Spm)含量、(Spd+Spm)/Put值及较低的Put/PAs值。说明耐盐品种“绿领特早”叶片具有较强的由游离态Put向游离态Spd和Spm转化的能力,维持了较低的游离态Put含量和较高的游离态Spd及Spm含量,进而抑制了活性氧过量积累。
Under the stress of 100mmol·L-1NaCl, two vegetable soybean cultivars (Glycine max (L.) Merr.] With different salt tolerance were used to grow dry matter, the yield per plant and the contents of free polyamines (PAs) levels were studied. The results showed that: NaCl stress significantly reduced the dry weight and yield per plant of vegetable soybean, but the decline rate of tolerant salt variety “LUCC” was lower than that of “salt sensitive variety” “95-1” Compared with “Ideal High Yield 95-1”, the leaves of “Lvlianzaozao” maintained relatively low H2O2 content during the whole NaCl stress. The contents of free putrescine and higher free spermidine (Spd), and the relatively high Spm content, (Spd + Spm) / Put value and lower Put / PAs value were maintained during 6-15 days of stress. The results showed that the salt-tolerance variety “LUCC” leaves had strong ability to convert from free Put to free Spd and Spm, maintained a lower free Put content and a higher free Spd and Spm content, Over-accumulation of reactive oxygen species.