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无论是在连续传代的过程中，还是在不同温度条件下保存的过程中，工程菌株ＷＨ－１均不稳定，其不稳定性主要表现在培养特征的变化和产生抗生素性能的衰退两个方面，且都是一个由量变逐渐转化为质变的过程。采用连续多次单孢子分离筛选的方法可以获得比较稳定的高产菌株。本实验主要通过５次单孢子分离筛选，得到了ＷＨ－１－１、ＷＨ－１－２、ＷＨ－１－３、ＷＨ－１－４和ＷＨ－１－５共５个较稳定的高产菌株
The results showed that the strain WH-1 was unstable during both continuous passage and storage under different temperature conditions. Its instability mainly manifested in two aspects: the change of culture characteristics and the decline of antibiotic performance, And all of them are a process of gradual transformation from quantitative change to qualitative change. Using continuous single spore separation screening method can be obtained relatively stable high-yielding strains. In this experiment, five isolates of single spore were screened, and five stable strains of WH-1-1, WH-1-2, WH-1-3, WH-1-4 and WH-