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目的:评价常用的4种梅毒血清学试验检测方法在临床诊断中的价值。方法:分别用快速血浆反应素试验(RPR)、胶体金免疫层析法(GICA)、酶联免疫吸附试验(ELISA)和微粒子化学发光法(CMIA)对264例标本进行梅毒血清学试验检测,同时与明胶颗粒凝集法(TPPA)的检测结果进行比较,得出不同检测方法的敏感度和特异性。结果:RPR、GICA、ELISA、CMIA的敏感度分别为67.5%、92.1%、96.0%和98.4%,特异性分别为89.1%、100%、100%和100%。结论:RPR可结合其他方法对梅毒进行联合诊断和对已诊断的患者进行疗效观察;GICA适合快速初筛,检测结果需结合其他方法进一步确认;ELISA和CMIA均可替代TPPA法作为临床诊断试验选用。
Objective: To evaluate the value of four common serological test methods for syphilis in clinical diagnosis. Methods: Totally 264 cases were tested for syphilis serology by RPR, GICA, ELISA and CMIA, respectively. At the same time, the results of gelatin particle agglutination assay (TPPA) were compared to get the sensitivity and specificity of different detection methods. Results: The sensitivity of RPR, GICA, ELISA and CMIA was 67.5%, 92.1%, 96.0% and 98.4% respectively, and the specificity was 89.1%, 100%, 100% and 100% respectively. Conclusion: RPR can be combined with other methods for the diagnosis of syphilis and the efficacy of the treatment of patients have been diagnosed; GICA suitable for rapid screening, test results need to be further confirmed by other methods; ELISA and CMIA alternative TPPA method as a clinical diagnostic test .