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目的:建立广地龙药材游离氨基酸类成分的HPLC指纹图谱分析方法。方法:采用2,4-二硝基氟苯柱前衍生化法,色谱柱为Kromasil C18分析柱(250 mm×4.6 mm,5μm),以乙腈(A)与N,N-二甲基甲酰胺-0.025 mol/L醋酸钠(1∶100,乙酸调pH6.0)(B)为流动相,梯度洗脱,在360 nm波长下检测,柱温为40℃,流速为1.0 mL/min。结果:对15个批次的广地龙药材进行了分析,确定了18个共有峰,不同批次药材相似度均大于0.87,建立了广地龙药材氨基酸类成分的HPLC指纹图谱。结论:建立了广地龙指纹图谱检测标准,为广地龙药材的鉴别和质量控制提供了依据。
OBJECTIVE: To establish a HPLC fingerprinting method for the determination of free amino acids in Radix et Rhizoma. METHODS: Pre-derivatization with 2,4-dinitrofluorobenzene was performed on a Kromasil C18 column (250 mm × 4.6 mm, 5 μm) with acetonitrile (A) and N, N-dimethylformamide -0.025 mol / L sodium acetate (1: 100, acetic acid adjusted to pH6.0) (B) as the mobile phase, gradient elution and detection at 360 nm with a column temperature of 40 ℃ and a flow rate of 1.0 mL / min. Results: Fifteen batches of Radix Pueraria lobata were analyzed and 18 common peaks were identified. The similarity of different batches of medicinal herbs was greater than 0.87. The HPLC fingerprint of amino acids in Radix isatidis was established. CONCLUSION: The establishment of the fingerprinting detection standard of Radix Codonopsis provides the basis for the identification and quality control of Radix Cynanchi panax.