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用３Ｈ－胸腺嘧啶核苷掺入实验及细胞计数法等观察了胰岛素对体外培养的人血管平滑肌细胞ＤＮＡ合成、细胞增殖及原癌基因：ｃ－ｆｏｓ和ｃ－ｍｙｃ表达的影响。结果发现，在培养基中加入不同浓度的胰岛素后，血管平滑肌细胞增殖，细胞ＤＮＡ合成增加，原癌基因ｃ－ｆｏｓ、ｃ－ｍｙｃ表达增加，上述三者均具有胰岛素剂量依赖性，呈现量－效关系．实验结果表明，高胰岛素血症可能通过促进平滑肌细胞增殖和原癌基因ｃ－ｆｏｓ和ｃ－ｍｙｃ表达和来加速动脉粥样硬化的发生与发展的。
The effects of insulin on the DNA synthesis, cell proliferation and the expression of proto-oncogenes c-fos and c-myc in cultured human vascular smooth muscle cells were observed with 3H-thymidine incorporation assay and cell counting method. The results showed that the proliferation of vascular smooth muscle cells, the increase of DNA synthesis and the increase of the expression of proto-oncogene c-fos and c-myc in the medium with different concentrations of insulin, all of which were insulin-dependent, Effective relationship. The experimental results show that hyperinsulinemia may accelerate the occurrence and development of atherosclerosis by promoting the proliferation of smooth muscle cells and the expression of protooncogene c-fos and c-myc.