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为提高反应系统中的底物浓度,减少酶用量,以蒸汽爆破预处理的玉米秸秆及高性能的酵母菌株Y5进行补料同步糖化发酵研究。通过同步糖化发酵策略的优化,经两次补料使反应体系底物浓度提高至25.7%,同步糖化发酵72h,即可获得41.7g/L的乙醇浓度,综合转化率64%。同时,纤维素酶及β-葡萄糖苷酶用量分别可降至7FPU/(g纤维素)及7IU/(g纤维素)。
In order to increase the substrate concentration in the reaction system and reduce the amount of enzyme, the simultaneous saccharification and fermentation of corn stover and high-performance yeast strain Y5 with steam explosion were studied. Through the optimization of the synchronous saccharification and fermentation strategy, the substrate concentration of the reaction system was increased to 25.7% after twice feeding, and the ethanol concentration of 41.7g / L was obtained after 72h of simultaneous saccharification and fermentation, with the overall conversion rate of 64%. At the same time, cellulase and β-glucosidase dosage can be reduced to 7FPU / (g cellulose) and 7IU / (g cellulose).