笔者在教学推广食用菌中 ,按传统常规制作试管母种成功率较低 ,经多次试验用木耳原基制母种成功率达 95 %。黄背木耳、黑木耳代料生产时做套圈出耳 ,耳基一形成就如乒乓球大小的原基。组织分离前选一最好的木耳原基取下放入接种箱内 ,勿用酒精消毒 ,用手术刀片轻轻削去外表层再 The author in teaching and promotion of edible mushrooms, according to the conventional production of test tube mother success rate is low, after many experiments with fungus primordial mother success rate of 95%. Yellow back fungus, black fungus production of ferrule ears, the formation of the ear base, such as the size of a table tennis base. Organize the pre-selected one of the best fungus primordium removed into the inoculation box, do not use alcohol disinfection, gently scalpel with a surgical blade to remove the outer surface and then