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目的:观察丹参酮ⅡA纳米制剂对大鼠全脑缺血再灌注损伤的保护作用.方法:采用三动脉夹闭法制备大鼠全脑缺血再灌注损伤模型,以丹参酮ⅡA纳米制剂灌胃给药,观察丹参酮ⅡA纳米制剂对脑组织的丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力和脑组织Na~+-K~+-ATPase活性水平的影响.做脑组织病理切片检查和透射电镜观察.结果:丹参酮ⅡA纳米制剂(25 mg/kg)能使脑组织MDA含量下降(P＜0.01),SOD活性明显升高(P＜0.05),Na~+-K~+-ATP活性显著上升(P＜0.01),病理切片和透射电镜观察显示丹参酮ⅡA纳米制剂能在不同的水平上抑制细胞和线粒体的损伤.结论:丹参酮ⅡA纳米制剂对全脑缺血再灌注损伤具有很好的保护作用.“,”AIM: To investigate the protection effect of preparation of nanometer Tanshinone II A against brain injury in rats undergone global cerebral ischemia reperfusion. METHODS: The rats were intragastrieal-ly administrated with the preparation of nanometer Tanshinone Ⅱ A, then the global cerebral ischemia-reperfu-sion model of rat was made by clipping and releasing three arteries. The content of MDA and the activity of SOD and Na~+ -K~+ -ATPase in homogenate of the cerebral tissues were measured. The sections of pathology and ultrastructure of mitochondrion in cortex had been observed. RESULTS: Tanshinone ⅡA (25 mg/kg) could obviously decrease the content of MDA (P < 0.01) and increase the activity of SOD(P < 0.05) and Na~+ -K~+ - ATPase (P < 0.05) in rats brain tissue. The observation of pathology and ultrastructure showed that the injury of cell and mitochondrion had been restrained in these of rat brain. CONCLUSION: Preparation of nanometer Tanshinone II A has protective effect against global cerebra injury caused by global cerebral ischemia-reperfusion injury in rats.