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目的:探讨炎症因子白介素(IL)-1β外膜介导小型猪冠状动脉粥样硬化病变及其可能机制。方法:小型雄性家猪16只随机分为2组,开胸手术分离冠状动脉左前降支和回旋支近端,对照组(n=8)血管外膜包裹吸附含生理盐水琼脂糖微粒悬液的纸巾;模型组(n=8)包裹吸附含IL-1β2.5μg琼脂糖微粒悬液的纸巾。2周后,冠状动脉造影观察管腔狭窄程度,光镜观察管腔病理学改变。结果:冠状动脉外膜包裹IL-1β血管段发生管腔狭窄,光镜可见病变血管段内膜增殖和炎症细胞聚集现象,外膜可见大量炎症细胞浸润。逆转录聚合酶链反应示模型组Rho激酶、单核细胞趋化蛋白-1、细胞间黏附分子-1和血管细胞间黏附分子-1mRNA表达强度高于对照组(P<0.05)。结论:IL-1β包裹冠状动脉外膜介导冠状动脉炎症反应及粥样硬化病变,上调细胞因子和黏附因子的表达是其主要作用途径之一。
Objective: To investigate the possible mechanism of coronary artery atherosclerosis in miniature pigs mediated by interleukin (IL) -1β, an inflammatory cytokine. Methods: Twenty-six male domestic pigs were randomly divided into two groups. Thoracotomy was performed to separate the left anterior descending coronary artery and proximal circumflex artery. The control group (n = 8) Paper towel; model group (n = 8) wrapped tissue paper containing IL-1β2.5μg agarose particle suspension. Two weeks later, the extent of stenosis was observed by coronary angiography and the pathological changes of lumen were observed by light microscope. Results: The lumen stenosis of IL-1β in the perivascular membrane was observed. The intimal hyperplasia and inflammatory cell aggregation in the diseased vascular segment were observed by light microscopy. A large number of inflammatory cells infiltrated the outer membrane. RT - PCR showed that Rho kinase, monocyte chemoattractant protein - 1, intercellular adhesion molecule - 1 and vascular cell adhesion molecule - 1 mRNA expression in model group were higher than those in control group (P <0.05). CONCLUSION: IL-1β is involved in the perivascular membrane-mediated coronary artery inflammation and atherosclerotic lesions. Upregulating the expression of cytokines and adhesion molecules is one of the main pathways.