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目的探讨纳米二氧化硅和纳米ZnO颗粒对人肝癌(HepG2)细胞毒性的影响。方法将处于对数生长期的细胞分别暴露于含终浓度分别为0(对照)~200μg/ml纳米二氧化硅、纳米ZnO颗粒的无血清培养基中培养24 h。检测细胞活性及细胞膜的完整性。结果与对照组比较,各浓度纳米二氧化硅及25~200μg/ml纳米ZnO染毒组HepG2细胞的存活率均较低,差异有统计学意义(P<0.05);且随着染毒浓度的升高,纳米二氧化硅和纳米ZnO染毒HepG2细胞的存活率均呈下降趋势。与相同浓度纳米二氧化硅相比,50~200μg/ml纳米ZnO染毒组HepG2细胞的存活率均较高,差异有统计学意义(P<0.05)。与对照组比较,各浓度纳米二氧化硅及纳米ZnO染毒组HepG2细胞上清液中LDH的活力均较高,差异有统计学意义(P<0.05);且随着纳米二氧化硅和纳米ZnO染毒浓度的升高,HepG2细胞上清液中LDH的活力均呈上升趋势。与相同浓度纳米二氧化硅相比,各浓度纳米ZnO染毒组HepG2细胞上清液中LDH的活力均较低,差异有统计学意义(P<0.05)。结论两种纳米颗粒均可对HepG2细胞产生毒性作用,且纳米二氧化硅比纳米ZnO颗粒的细胞毒性作用更强。
Objective To investigate the effects of nano-silica and nano-ZnO particles on the cytotoxicity of HepG2 cells. Methods Cells in logarithmic growth phase were exposed to serum-free medium containing nano-silica and nano-ZnO with the final concentration of 0 (control) ~ 200μg / ml for 24 hours respectively. Cell viability and cell membrane integrity were examined. Results Compared with the control group, the survival rates of HepG2 cells treated with nanosilica and 25 ~ 200μg / ml ZnO were lower than those of the control group (P <0.05). With the concentration of Increased, the survival rate of HepG2 cells exposed to nano-silica and nano-ZnO all showed a decreasing trend. Compared with the same concentration of nano-silica, the survival rate of HepG2 cells in 50 ~ 200μg / ml nano-ZnO was higher than that in the same concentration (P <0.05). Compared with the control group, the activities of LDH in the supernatant of HepG2 cells in each concentration of nano-silica and nano-ZnO were higher than that in the control group (P <0.05). With the increase of nano-silica and nano The concentration of ZnO increased the activity of LDH in HepG2 supernatant. Compared with the same concentration of nano-silica, the activity of LDH in the supernatant of HepG2 cells in each concentration of nano-ZnO was lower than that in the same concentration (P <0.05). Conclusion Both nanoparticles can produce toxic effects on HepG2 cells, and the cytotoxicity of nano-silica particles is stronger than that of nano-ZnO particles.