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对已筛选到的人源性抗ＨＢｓＡｇ特异性噬菌体抗体基因文库进行表达研究，将特异性噬粒转化大肠杆菌ＸＬ１－Ｂｌｕｅ，反复冻溶法制备可溶性Ｆａｂ片段。免疫印迹实验表明大肠杆菌质周腔内有可溶性Ｆａｂ片段的表达。制备羊抗人ＩｇＧＦａｂ抗血清、建立抗人ＩｇＧＦａｂ抗体－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析柱，纯化Ｆａｂ片段。ＳＤＳ－ＰＡＧＥ显示纯化的Ｆａｂ达免疫纯，点印迹法表明纯化后的Ｆａｂ片段具有中和ＨＢｓＡｇ的活性
The screened human anti-HBsAg specific phage antibody gene library expression studies, the specific phagemid transformed into E. coli XL1-Blue, prepared by repeated freeze-thaw soluble Fab fragments. Western blotting experiments showed that E. coli peritumoral cavity soluble Fab fragment expression. Preparation of goat anti-human IgGFab antiserum, the establishment of anti-human IgGFab antibody Sepharose 4B affinity chromatography, Fab fragment purification. SDS-PAGE showed that the purified Fab was immunoprecipitated, and dot blot showed that the purified Fab fragment has the activity of neutralizing HBsAg