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[目的]探讨逆萎康对慢性萎缩性胃炎(chronic atrophic gastritis,CAG)大鼠胃黏膜的保护作用。[方法]将60只健康雄性Wistar大鼠随机分为空白对照组、模型组、逆萎康高、中、低剂量干预组及替普瑞酮干预组,每组10只。模型组给予幽门螺杆菌(Hp)悬液和乙醇-水杨酸钠溶液造模14周,逆萎康干预组、替普瑞酮干预组在造模的同时分别给予逆萎康、替普瑞酮干预治疗14周,空白对照组仅给予等量0.9%氯化钠灌胃14周。观察各组大鼠胃黏膜大体形态和组织学表现,检测各组大鼠胃黏膜中氨基己糖的含量,并应用Elisa法测定各组大鼠一氧化氮(NO)、超氧化物歧化酶(SOD)及Bcl-2的含量。[结果]逆萎康干预组、替普瑞酮干预组中NO、SOD、氨基己糖含量均较模型组大鼠高(P<0.05);Bcl-2均较模型组低(P<0.05)。而逆萎康干预组NO、SOD、Bcl-2及氨基己糖含量与替普瑞酮干预组比较差异无统计学意义(P>0.05)。[结论]逆萎康与替普瑞酮一样对CAG大鼠的胃黏膜具有保护作用,可能是通过增加NO、SOD、氨基己糖含量、降低Bcl-2含量实现的。
[Objective] To explore the protective effect of Wei Wei Kang on gastric mucosa in rats with chronic atrophic gastritis (CAG). [Methods] Sixty healthy male Wistar rats were randomly divided into blank control group, model group, Wulixang high, medium and low dose intervention group and teprenone intervention group, with 10 rats in each group. The model group was given H. pylori (Hp) suspension and ethanol-sodium salicylate solution for 14 weeks. The rats in the anti-Wulcan intervention group and the teprenone-treated group were treated with anti-Wulkang and Tipri Treatment of ketone for 14 weeks, the blank control group was given only 0.9% sodium chloride for 14 weeks. The morphological and histological changes of gastric mucosa of rats in each group were observed. The contents of hexosamine in gastric mucosa in each group were measured. The levels of nitric oxide (NO) and superoxide dismutase SOD) and Bcl-2 content. [Results] Compared with the model group, the content of NO, SOD and hexosamine in the group treated with Weiweikang and Teprenone were significantly higher than those in the untreated group (P <0.05); Bcl-2 was lower (P <0.05) . However, the levels of NO, SOD, Bcl-2 and hexosaminoglycan in the intervention group were not significantly different from those in the intervention group (P> 0.05). [Conclusion] Gu Wei Kang and Teprenone have the same protective effect on gastric mucosa in CAG rats, which may be achieved by increasing the contents of NO, SOD, hexosamine and Bcl-2.