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为了解不同寄主植物对亚洲玉米螟Ostrinia furnacalis(Guenée)寄主专化性及遗传多样性的影响,以东北地区取食4种寄主植物的亚洲玉米螟种群为研究对象,选取其线粒体COⅠ和COⅡ基因作为分子标记,通过序列比对分析,研究了亚洲玉米螟种群间的遗传多样性、基因流水平及分子变异。结果显示,亚洲玉米螟线粒体基因具有较丰富的遗传多态性;COⅠ和COⅡ基因总寄主植物种群Fu’s Fs检验结果分别为-1.82和-2.04,种群历史呈扩张趋势,基因流Nm分别为6.38和3.24,说明各种群间基因交流水平较高;遗传变异主要来自于种群内部;亚洲玉米螟的单倍型在BI树和NJ树上的分布拓扑结构与寄主植物间无对应关系。研究表明,取食不同寄主植物的亚洲玉米螟种群间的遗传多态性较高,基因交流频繁,且尚未发生遗传分化。
In order to understand the effects of different host plants on host specificity and genetic diversity of Ostrinia furnacalis (Guenée), four populations of A. trituberculatus in northeastern China were selected as study objects, and their mitochondrial COⅠ and COⅡ genes As a molecular marker, the genetic diversity, gene flow and molecular variation among Asiatic corn borer populations were studied by sequence alignment. The results showed that mitochondrial gene of Asian corn borer had rich genetic polymorphism; Fu's Fs test results of total host plant population of COⅠ and COⅡ gene were -1.82 and -2.04, respectively. The population history showed a trend of expansion with gene flow Nm of 6.38 and 3.24, indicating a high level of gene exchange among the populations. The genetic variation mainly came from the inner part of the population. There was no correlation between the distribution topology of the Asian corn borer and the host plants in the BI and NJ trees. Studies have shown that the genetic diversity among populations of Asiatic corn borer (Oryza sativae) fed on different host plants is high, with frequent gene exchanges and no genetic differentiation.