论文部分内容阅读
在酶免疫转移印渍技术（EITB）分析斯氏肺吸虫虫体成份，显示10～30KD抗原蛋白（主带22、24和26KD）对该病具免疫诊断价值的基础上，将SDS－PAGE和电洗脱技术相结合，分离并纯化出斯氏肺吸虫成虫的10～30KD蛋白组份，采用Dot－ELISA方法免疫诊断肺吸虫。10～30kD的斯氏肺吸虫成虫抗原与斯氏、卫氏肺吸虫病人血清均呈阳性反应，与其它吸虫病和健康人血清末产生反应。该结果进一步表明10～30KD斯氏肺吸虫成虫抗原的Dot－ELISA为肺吸虫病高度特异、敏感的免疫诊断方法。并为两种肺吸虫的共同抗原，可用于免疫诊断斯氏、卫氏肺吸虫病。
SDS-PAGE and SDS-PAGE were used to analyze the immunogenicity of the parasites from 10 ~ 30 kD antigen proteins (22, 24 and 26 kD) by immunoblotting with enzyme-linked immunosorbent assay (EITB) Electro-eluting technique was used to isolate and purify the 10-30KD protein components of adult Paragonimus paraguastris. Dot-ELISA was used to immunodiagnosis Paragonimus. 10 ~ 30kD Paragonimus adult worms antigens and Schistosoma & apos; s Paragonimus patients serum positive reaction, and other chelonia and healthy people at the end of the reaction. The results further indicate that the Dot-ELISA of 10-30 KD adult worms antigen is a highly specific and sensitive immunodiagnosis method for paragonimiasis. And the common antigen of two kinds of paragonimiasis, can be used for immune diagnosis of schistosomiasis, paragonimiasis.