论文部分内容阅读
为确定从小麦加工废水中提取水不溶性戊聚糖的最佳工艺,首先以固液比、提取时间、温度、碱浓度等条件进行单因素实验,并在此基础上进行4因素3水平的响应面设计,以戊聚糖得率为响应值进行响应面分析,进一步优化提取工艺条件。结果表明:NaOH浓度为0.6 mol/L,提取温度为64.68℃,固液比为1:18.58,提取时间为3.18 h,提取率达到73.5792%。接下来还对得到的产物进行相对分子量分析,采用阴离子交换柱DEAE-纤维素层析柱纯化戊聚糖,分别采用蒸馏水和NaCl溶液为洗脱剂纯化,并用Sphroase-CL 6B层析柱分析,结果显示,水洗脱的戊聚糖相对分子量为1730 ku;NaCl溶液洗脱的戊聚糖相对分子量为1170 ku。此项研究为后续降低水不溶性戊聚糖相对分子量从而提高其可溶性奠定基础。
In order to determine the optimum process for extracting water-insoluble pentosan from wheat processing waste water, single-factor experiments were conducted on the basis of solid-liquid ratio, extraction time, temperature and alkali concentration, and then the response of 4 factors and 3 levels Surface design, response rate analysis of the pentosan yield, to further optimize the extraction process conditions. The results showed that the optimum conditions were: NaOH concentration 0.6 mol / L, extraction temperature 64.68 ℃, solid - liquid ratio 1: 18.58, extraction time 3.18 h and extraction rate 73.5792%. Then, the relative molecular weight of the obtained product was analyzed. Pentosan was purified by anion exchange column DEAE-cellulose column, purified by distilled water and NaCl solution respectively and analyzed by Sphroase-CL 6B column. The results showed that the relative molecular weight of pentosan extracted by water was 1730 ku and that of pentosan extracted by NaCl solution was 1170 ku. This study lays the foundation for subsequent reduction of the relative molecular weight of water-insoluble pentosans to increase their solubility.