脑外伤患者血清NSE、Tau、S100、MMP-9水平与脑水肿相关性的研究

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[目的] 探讨脑外伤患者血清神经烯醇化酶(NSE)、Tau蛋白、S100蛋白、基质金属蛋白酶9(MMP-9)与脑水肿的相关性.[方法] 选择2013年1月至2016年11月本院收治的96例颅脑外伤患者的临床资料,将其分为观察组,选择同期招募的60名健康者作为对照组,脑外伤患者于入院时、入院后d3和d7分别行头部CT检查评估脑水肿体积.采用ELISA法检测脑外伤患者入院当日的血清NSE、Tau、S100、MMP-9蛋白水平,所有健康对照在入选后亦检测相同指标.比较两组患者的血清NSE、Tau、S100、MMP-9蛋白水平,分析观察组脑水肿体积与各血清蛋白Pearson相关性.[结果] 观察组患者入院当日血清NSE、S100、MMP-9水平均高于对照组,差异均具有统计学意义(均P0.05).入院时观察组患者的血清NSE、Tau、S100、MMP-9水平均与入院当天CT检测的脑水肿体积呈显著正相关性(均P<0.05);入院时血清S100、MMP-9水平与入院d3的脑水肿体积亦呈显著正性相关性(均P<0.05).入院时血清S100、MMP-9水平与脑水肿进展值呈显著正相关性.[结论] 血清NSE、Tau、S100和MMP-9水平可在一定程度上反映脑外伤后脑水肿的程度,并且MMP-9、S100还可提示脑水肿的进展可能,对评估患者受伤后脑水肿的情况具有参考价值.“,”[Objective]To investigate the correlation between serum neuron specific enolase (NSE), Tau protein, S100 protein, matrix metalloproteinase 9 (MMP-9) and brain edema in patients with traumatic brain injury (TBI).[Methods]Clinical data of 96 patients with traumatic brain injury admitted in our hospital from January 2013 to November 2016 were selected and divided into observation group, and the healthy volunteers were recruited as control group in the same period of time as the control group (n =60), the brain injury patients were evaluated by head CT examination of brain edema volume at the admission, d3 and d7 after admission.ELISA method was used to detect the serum NSE, Tau, S100, MMP-9 protein levels in the patients with traumatic brain injury , and all the healthy controls were tested for the same index.The serum levels of NSE, Tau, S100 and MMP-9 protein were compared between the two groups, and the correlation between the volume of brain edema and serum protein Pearson was analyzed.[Results]The serum NSE, S100 and MMP-9 in the observation group were higher than those in the control group on the day of admission, the difference was statistically significant (mean P0.05).The serum NSE, Tau, S100, MMP-9 levels in the observation group were significantly positively correlated with the volume of brain edema (through CT exam) on the day of admission (mean P<0.05);The level of serum S100, MMP-9 and the volume of brain edema in admission d3 were significantly positive (P<0.05).There was a significant positive correlation between serum S100, MMP-9 level and the value development of brain edema.[Conclusion]Serum NSE, Tau, S100 and MMP-9 could reflect the severity of brain edema in TBI, and both MMP-9 and S100 may predict the progress of brain edema, which are valuable in evaluation of the brain edema of TBI patients.
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