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一次皮下注射惊厥剂量（7．5mg／kg）的红藻氨酸（kainicacid，KA），诱发Fisher344大鼠出现急性癫痫发作，7d后即可形成癫痫敏感大鼠。继用Gelshift、Super－shift和Westernblot方法测定大鼠海马内AP－1DNA结合活性及其组成成分。Gelshift结果显示，癫痫敏感大鼠海马内AP－1DNA结合活性的基础水平较对照组为高；Super-shift实研表明仅Fra和JunD抗体能把AP－1蛋白滞留到较高的位置；Westernblot进一步证明JunD蛋白包括43，39and28kDaJund；又癫痫敏感大鼠若第二次接受癫痫刺激，其AP－1复合物的活性及成分均再次发生改变。以上资料提示AP－1DNA结合活性的增高和高水平的JndD很可能在癫痫敏感性长期持续增高的维持中起重要作用。
A subcutaneous injection of convulsant dose (7.5mg / kg) of kainic acid (kainicacid, KA) induced Fisher344 rats with acute epileptic seizures, 7d after the formation of epilepsy-sensitive rats. The binding activity of AP-1 DNA in rat hippocampus and its components were determined by Gelshift, Super-shift and Western blotting. Gelshift results showed that basal level of AP-1 DNA binding activity in hippocampus of epileptic-sensitive rats was higher than that in control group. Super-shift assay showed that only Fra and JunD antibodies could retain AP-1 protein in higher position. Western blot Proved JunD protein including 43, 29and 28kDaJund; and epilepsy-sensitive rats if the second epileptic stimulation, AP-1 complex activity and composition were changed again. The above data suggest that increased AP-1 DNA binding activity and high levels of JndD are likely to play an important role in the maintenance of long-term sustained increase in seizure susceptibility.