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甜瓜坏死斑点病毒(Melon necrotic spot virus,MNSV)是我国甜瓜病毒新纪录种,主要靠种子和土壤真菌传播。本文以MNSV山东分离物(MNSV-Shandong)为材料,利用RT-PCR和RACE技术获得了MNSV山东分离物基因组,并分析了其基因组成和进化关系。MNSV-Shandong基因组为4 267 nt,编码5个ORF,参与复制的p29和p89基因分别编码29 kDa和89 kDa蛋白质;p42基因编码的外壳蛋白为42 kDa;p7A和p7B基因编码的移动蛋白均为7 kDa。系统进化树表明MNSV-Shandong与海门分离物(MNSV-HM)聚在一起,与欧洲、部分亚洲及北美洲在一个分支上,与日本分离物距离较远。本文为国内首次报道MNSV山东分离物基因组。
Melon necrotic spot virus (MNSV) is a new record of melon virus in China, and mainly spreads by seeds and soil fungi. In this paper, the MNSV Shandong isolate (MNSV-Shandong) as the material, the use of RT-PCR and RACE technology was obtained MNSV Shandong isolates genome, and analysis of its genetic composition and evolutionary relationship. The genome of MNSV-Shandong was 4 267 nt, encoding 5 ORFs. The p29 and p89 genes involved in the replication encoded 29 kDa and 89 kDa proteins respectively. The coat protein encoded by p42 gene was 42 kDa. The coding proteins of p7A and p7B genes were 7 kDa. The phylogenetic tree indicated that MNSV-Shandong and Haimen isolates (MNSV-HM) clustered together, and were distant from Japanese isolates on a branch with Europe, part of Asia and North America. This is the first report of the MNSV Shandong isolates genome.