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目的:为了检测活性维生素D缺乏及其母乳成分的改变是否影响小鼠下颌骨以及牙齿的发育。方法:通过采用X线摄影、微CT和组织学等方法比较分析25羟维生素D3的1α羟化酶杂合子[1α(OH)ase+/-]和纯合子[1α(OH)ase-/-]母鼠所喂养的纯合子和杂合子小鼠下颌骨以及牙齿的表型差异。结果:母乳中的钙水平在1α(OH)ase-/-母鼠较1α(OH)ase+/-母鼠为低。1α(OH)ase-/-仔鼠的牙量和骨量较同窝1α(OH)ase+/-仔鼠明显下降,而由1α(OH)ase-/-母鼠所喂养仔鼠的牙量和下颌骨骨量比由1α(OH)ase+/-母鼠喂养的仔鼠要低。由1α(OH)ase+/-母鼠所喂养的1α(OH)ase+/-仔鼠牙量和下颌骨量最高,而由1α(OH)ase-/-母鼠所喂养的1α(OH)ase-/-仔鼠牙量和下颌骨量最低。结论:活性维生素D和高乳钙摄入各自具有刺激下颌骨和牙齿矿化和发育的作用,又具有协同作用。
OBJECTIVE: To test whether changes in active vitamin D deficiency and breast milk affect mouse mandibular and tooth development. Methods: The effects of 1α (OH) ase +/-] and homozygous [1α (OH) ase - / -] on 25-hydroxyvitamin D3 were compared by using X-ray photography, The differences in the mandibular and dental phenotypes of homozygous and heterozygous mice fed by female rats. Results: Calcium levels in breast milk were lower in the 1α (OH) ase - / - than in the 1α (OH) ase +/- mice. Dental calculus and bone mass decreased significantly in 1α (OH) ase - / - offspring compared with that in littermate 1α (OH) ase +/- offspring, whereas teeth fed off by 1α (OH) ase - / - Was lower than that of the offspring with mandibular bone mass ratio of 1α (OH) ase +/-. 1α (OH) ase +/- mice fed 1α (OH) ase +/- mice had the highest amounts of teeth and mandibles, whereas the 1α (OH) ase mice fed 1α (OH) ase - / - - / - The lowest amount of teeth and mandible in the offspring. CONCLUSIONS: Both active vitamin D and high-calcium intake have a synergistic effect on mineralization and development of the mandible and teeth.