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目的：探讨ＥＢ病毒感染与齐齐哈尔地区鼻咽部淋巴瘤的关系。方法：常规染色后行组织学分类，利用免疫组化ＬＳＡＢ法确定肿瘤的免疫表型。采用原位杂交方法检测ＥＢ病毒编码的小核ＲＮＡ（ＥＢＥＲｓ）。结果：２１例鼻咽部淋巴瘤中，Ｔ细胞淋巴瘤１７例，占８０９％其中高度恶性１５例，均为中～大Ｔ细胞淋巴瘤；低度恶性２例，为小Ｔ细胞淋巴瘤。Ｂ细胞淋巴瘤４例，高度恶性３例，分别为中心母细胞性淋巴瘤和免疫母细胞性淋巴瘤；低度恶性１例，为浆细胞性淋巴瘤。所检病例中，１７例Ｔ细胞淋巴瘤中１１例ＥＢＶ（６４７％）阳性，４例Ｂ细胞淋巴瘤均为阴性。阳性信号位于肿瘤细胞核内，呈深蓝色，分布于１０％～８０％的瘤细胞核内。阳性病例均为中～大Ｔ细胞淋巴瘤，都有血管浸润，２例伴有明显坏死。结论：该地区鼻咽部Ｔ细胞淋巴瘤与ＥＢ病毒的感染有关。
Objective: To investigate the relationship between EB virus infection and nasopharyngeal lymphoma in Qiqihar area. METHODS: Histological classification was performed after routine staining. The immunohistochemical LSAB method was used to determine the immunophenotype of the tumor. In situ hybridization was used to detect EB virus-encoded small nuclear RNA (EBERs). RESULTS: Twenty-seven cases of nasopharyngeal lymphoma had T-cell lymphoma in 17 cases, accounting for 80.9% of them. Among them, 15 cases were highly malignant, all of them were medium to large T-cell lymphomas, and 2 cases of low-grade malignancies were small T-cell lymphoids. tumor. There were 4 cases of B-cell lymphoma and 3 cases of high-grade malignancies, which were central blastoblastic lymphoma and immunoblastic lymphoma. One case of low-grade malignancy was plasma cell lymphoma. Among the cases examined, 11 cases of T-cell lymphoma were positive for EBV (64.7%), and 4 cases of B-cell lymphoma were negative. The positive signal is located in the nucleus of tumor cells and is dark blue. It is distributed in the nucleus of tumor cells ranging from 10% to 80%. The positive cases were all medium to large T-cell lymphomas, with vascular infiltration, and 2 cases with marked necrosis. CONCLUSIONS: Nasopharyngeal T-cell lymphoma in this region is associated with Epstein-Barr virus infection.