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本文报告了用半胱氨酸、2-巯基丙醇、深铜磺酸盐(BCS)替代饲养细胞进行体外培养卡氏肺孢子虫的方法。用D-MEM或L-15培养基,培养基中含10％胎牛血清(FBS)、1000U/ml青霉素、1000μg/ml链霉素、0.5μg/ml两性霉素B、100μM2-巯基丙醇、50μM BCS,将生长5周的雌性先天性无胸腺裸鼠(BALB/c nu/nu)与卡氏肺孢子虫感染裸鼠关在一个笼子里,经过几周适应之后,处死感染鼠,将整个肺切除,制成匀浆,用无菌网纱滤过匀浆,将滤液离心,用0.83％氯化铵处理以溶解红细胞,用磷酸盐缓冲液反复冲洗,移出上清液,将微
In this paper, a method of in vitro cultivation of Pneumocystis carinii using cysteine, 2-mercaptopropanol, deep copper sulfonate (BCS) instead of feeder cells was reported. The cells were cultured in D-MEM or L-15 medium containing 10% fetal bovine serum (FBS), 1000 U / ml penicillin, 1000 μg / ml streptomycin, 0.5 μg / ml amphotericin B, 100 μM 2-mercaptopropanol , 50 μM BCS, female congenital athymic nude mice (BALB / c nu / nu), which grew for 5 weeks, were placed in a cage with nude mice infected with Pneumocystis carinii. After several weeks of acclimation, infected mice were killed The whole lung is resected, homogenized, homogenized with sterile gauze, the filtrate is centrifuged, treated with 0.83% ammonium chloride to dissolve erythrocytes, repeatedly rinsed with phosphate buffer, the supernatant removed,