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目的利用乙二胺四乙酸二钠盐(EDTA-Na2)体外脱钙模拟椎体骨质疏松,测量猪3联椎体骨密度和生物力学的特性,探讨一种短期制造骨质疏松性椎体压缩骨折模型的方法。方法取49个新鲜猪胸腰椎3联体,随机分成7组,记为A.B.C.D.E.F.G每组7个标本,然后把B.C.D.E.F.G标本侵入0.4916 mmol/L的EDTA-Na2脱钙液体中,分别在0、5、7、9、11、13、15天分别行A.B.C.D.E.F.G骨密度、生物力学、Mcro-ct检查及每组取一个标本大体剖开。结果随着脱钙时间的延长,大体观察可见各组标本骨小梁逐渐变细、间隙增宽,骨密度检测结果显示逐渐减低的趋势,并且各组脱钙前后骨密度变化均有明显的差异性(P=0.0000)。随着脱钙时间的延长椎体压缩强度逐渐降低,脱钙前后相比压缩强度均有显著性差异(P=0.0000)。Micro-CT检测脱钙13天椎体及棘突骨矿物质密度、组织骨密度、骨体积分数、结构模型指数、欧拉数、骨小梁连结密度均有明显的变化(P≤0.02)。结论采用0.4916 mmol/L EDTA-Na2脱钙11～13天可模拟中、重度骨质疏松性椎体压缩骨折,是一种快速、可行、重复性高的方法。
Objective To evaluate the bone density and biomechanical characteristics of 3-vertebral body of vertebral osteoporosis by EDTA-Na2 decalcification in vitro and to explore a short-term osteoporotic vertebral body Compression fracture model approach. Methods Thirty-four fresh pig thoracolumbar spine triad bodies were randomly divided into 7 groups, each group was divided into 7 groups. ABCDEFG specimens were then invaded into 0.4916 mmol / L EDTA-Na2 decalcification liquid, ABCDEFG bone mineral density, biomechanics, Mcro-ct 7,9,11,13,15 days were taken and each group took a specimen roughly cut open. Results With the extension of decalcification time, trabecular bone was gradually thinned and the gap broadened. The results of bone mineral density showed a trend of decreasing gradually, and the changes of bone mineral density in all groups before and after decalcification were significantly different (P = 0.0000). With the decalcification time prolonged compressive strength decreased, before and after decalcification compared compressive strength were significantly different (P = 0.0000). Micro-CT examination of 13 days of decalcification vertebral body and spine bone mineral density, bone mineral density, bone volume fraction, structural model index, Euler number, trabecular bone connection density were significantly changed (P≤0.02). Conclusion Decalcification with 0.4916 mmol / L EDTA-Na2 for 11 ~ 13 days can simulate moderate and severe osteoporotic vertebral compression fractures, which is a rapid, feasible and highly reproducible method.