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目的:研究帕病2号方对帕金森病(PD)模型大鼠的抗氧化作用。方法:采用6-羟基多巴胺(6-OHDA)纹状体两点注射法造成左侧纹状体损毁模型,ip阿朴吗啡(APO)诱导并诊断大鼠旋转行为,将造模成功大鼠随机分为帕病2号方高、中、低剂量组(32.0,16.0,8.0 g·kg-1)、美多巴组(0.075 g·kg-1)、模型组,连续ig 4周,同时设立正常组,正常组与模型对照组给予等容积蒸馏水;应用比色法测定大鼠纹状体匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性及丙二醛(MDA)的含量,ip APO诊断大鼠旋转行为的变化。结果:神经行为学方面,模型组大鼠治疗前后旋转圈数无显著差异,帕病2号方高、中剂量组大鼠旋转圈数较模型组显著减少(P<0.05),且治疗前后比较差异显著(P<0.05);美多巴组大鼠旋转圈数亦明显减少(P<0.05)。模型组MDA(13.48±3.71)nmol·mg-1明显升高,GSH-Px,SOD(497.48±72.61)NU·g-1,(113.11±8.08)U·mg-1明显降低,与正常对照组相比差异显著(P<0.05或P<0.01);帕病2号方高、中剂量组可明显降低MDA(9.29±3.25),(9.62±2.78)nmol·mg-1,提高GSH-Px,SOD活性[分别为(612.53±53.20),(637.03±133.21)NU·g-1及(123.93±9.84),(120.21±10.63)U·mg-1](P<0.05);低剂量组仅GSH-Px增加(573.19±54.86)NU·g-1,MDA,SOD则无显著改变。美多巴组上述指标均无显著改善。结论:帕病2号方明显改善PD模型大鼠的旋转行为,提高其抗氧化能力和清除自由基的能力,并呈明显的量效关系。
OBJECTIVE: To study the anti-oxidative effect of Paseng No. 2 on Parkinson’s disease (PD) model rats. Methods: The left striatum damage model was induced by 6-hydroxydopamine (6-OHDA) striatum. The apomorphine (APO) induced and diagnosed the rat rotation behavior. The rats were randomized The rats were divided into two groups: high, medium and low doses of PASD 2 (32.0,16.0 and 8.0 g · kg -1), metoprolol group (0.075 g · kg -1), model group, continuous ig for 4 weeks The normal group, the normal group and the model control group were given distilled water of equal volume. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the striatum homogenate of rats were measured by colorimetry And malondialdehyde (MDA) content, ip APO diagnosis of rat rotation behavior changes. Results: In neurobehavioral aspects, there was no significant difference in the number of rotations before and after treatment in model group rats. The number of rotations of rats in model group and control group was significantly decreased compared with model group (P <0.05) (P <0.05). The number of rotations of the rats in the meridional group was also significantly reduced (P <0.05). The level of MDA (13.48 ± 3.71) nmol · mg-1 in the model group was significantly higher than that in the normal control group (GSH-Px, SOD, 497.48 ± 72.61, NU · g- (P <0.05 or P <0.01). P <0.01 or P <0.01), P <0.01 or P <0.01 could significantly decrease the levels of GSH-Px, (612.53 ± 53.20), (637.03 ± 133.21) NU · g-1 and (123.93 ± 9.84) and (120.21 ± 10.63) U · mg-1, respectively -Px increased (573.19 ± 54.86) NU · g-1, MDA, SOD no significant change. There was no significant improvement in the above indicators for the Madobal Group. Conclusion: Paoye 2 Prescription significantly improves the rotation behavior of rats with PD and enhances its antioxidant capacity and free radical scavenging capacity, and shows a significant dose-response relationship.