以水蛭素活性片段Ｈｉｒｕｄｉｎ５５～６４为模板，设计合成了３个含Ｎ－取代甘氨酸（ＮＳＧ）的化合物．其中氨基酸的连接采用多中心同步技术的标准肽合成法，ＮＳＧ的引入用亚单体合成路线，通过溴乙酰化和伯氨取代两步反应完成．所得化合物经高效液相色谱及质谱分析确证分子量． Hirudin 55 ~ 64, a hirudin active fragment, was used as a template to design and synthesize three compounds with N-substituted glycine (NSG). Among them, the amino acid linkage was based on the standard peptide synthesis method of multisite synchronization technology. The NSG was introduced into the synthesis route by the subunit, and the reaction was completed by two steps of bromoacetylation and primary amino substitution. The resulting compounds were confirmed by high performance liquid chromatography and mass spectrometry molecular weight.